Supporting Information DNA nanomachine-based intelligent logic controlled release systems

Tetraethoxysilane (TEOS, 28%), n-cetyltrimethylammonium bromide (CTAB, ≥99%), ammonium hydroxide solution (25%), 1-ethyl-3-(3-dimethylaminopropyl) carbodiimide (EDC, 99%), N-Hydroxysuccinimide (NHS, 98%), 3-Aminopropyltriethoxy-silane (APTES, 99%), succinic anhydride (99%), sodium citrate tribasic dihydrate, tannic acid, and Rhodamine B were purchased from Sigma Company. Hydrogen tetrachloroaurate (III) was purchased from Jinke reagent company, China. All DNAs were purchased from TaKaRa Biotech (Dalian, China) and used as received. All buffers were prepared with ultra-pure MilliQ water (resistance > 18 MΩ cm). Scanning electron microscopy (SEM) was performed with a JEOL-6700FE instrument. Transmission electron microscopy (TEM) images were obtained using a Philips CM 200 kV electron microscope. Powder X-ray diffraction (XRD) patterns were collected using a Rigaku D/max 2500 equipped with Cu Kα radiation. UV-Vis spectra were collected using a Hitachi U-4100 spectrophotometer. Fourier transform infrared (FT-IR) spectra were taken on a Bruker-EQUINOX55 spectrometer. All spectra were taken with an instrument resolution of 4 cm. All fluorescence spectra were recorded on a Hitachi F-4500 FL Spectrophotometer in PBS buffer. N2 adsorption-desorption isotherms were obtained at 77 K on a Micromeritics ASAP2020 automated sorption analyzer. The BET model was applied to evaluate the specific surface areas. Pore size and pore volume were determined from the adsorption data by the BJH method. CD spectra were collected on a JASCO J-810 CD spectrometer.